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            Dionex AminoPac PA1 氨基酸分析色譜柱

            更新時(shí)間:2019-11-12

            簡要描述:

            Dionex AminoPac PA1 氨基酸分析色譜柱
            特別適用于分析酸性和酸不穩(wěn)定性氨基酸,以及用陽離子交換分離不*的氨基酸對(duì)。

            Dionex AminoPac PA1 氨基酸分析色譜柱

            用途和特點(diǎn)
            AminoPac PA1柱用于氨基酸的快速分離。特別適用于分析酸性和酸不穩(wěn)定性氨基酸,以及用陽離子交換分離不*的氨基酸對(duì)。

             

             

            Dionex AminoPac PA1 氨基酸分析色譜柱

            訂貨信息:

            分析柱
            AminoPac PA1 Analytical Column (4 x 250 mm)037022
            保護(hù)柱
            AminoPac PA1 Guard Column (4 x 50 mm)037023

             

             

            For Specialized Amino Acid Applications with Postcolumn Derivatization

            The AminoPac® PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

            • Resolves phosphorylated and other amino acids by anion-exchange chromatography

            • Separates acid-labile amino acids under basic pH conditions

            • Separates strongly acidic amino acids

            • Requires OPA or ninhydrin postcolumn derivatization

            The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

            The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

             

             

             

            AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization  Specifications
            DimensionsAminoPac PA1 Analytical Column:
            4 × 250 mm
            AminoPac PA1 Guard Column:
            4 × 50 mm
            Maximum operating pressure4000 psi (27.9 MPa)
            Recommended operating temperatureAmbient
            Typical operating conditions1.0 mL/min gradient
            Recommended flow rate: 1.0 mL/min
            Mobile phase compatibilityCompatible with up to 5% common organic solvents.
            Recommended eluents: hydroxide, borate, and acetate.
            Resin composition10-μm-diameter, pellicular substrate (2% cross-linked)
            agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
            Anion-exchange capacityN/A
            Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
            All components are nonmetallic.

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